沈阳医学院 · 辽宁 · PDF · 13 页 · 4103KB
Small Methods www.small-methods.com RESEARCH ARTICLE Bio-Inspired Laccase-Mimicking Cu3 –MOF Nanozyme as Colorimetric Sensor Array for Rapid Degradation and Visual Sensing of Phenolic Compounds Haitao Han1 YangCui Qu1 Xinhe Duan2 JunHao Cui3 Li Song1 Hao Wang1 Jie Yang1 Guannan Wang2 1 College of Medical Engineering& the Key Laboratory for Medical Functional Nanomaterials Jining Medical University, Jining, Shandong, P. R. China 2 School of Pharmacy, Shenyang Medical College, Shenyang, Liaoning, P. R. China 3 Department of Orthopaedics, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan, Shandong, P. R. China Correspondence: Guannan Wang ( chemwangguannan@symc.edu.cn) Received: 3 November 2025 Revised: 19 December 2025 Accepted: 21 December 2025 Keywords: chemical stability | colorimetric sensor array | nanozym | phenolics | trinuclear copper ABSTRACT Trinuclear copper complexes that emulate the active sites of multicopper oxidases (MCOs) are of broad biological interest. Here, a monoatomic-node strategy combined with solvent reduction was used to construct a rare trinuclear copper MOF featuring a planar, equilateral-triangular CuI 0.6 CuII 2.4 core. The CuI 0.6 CuII 2.4 –MOF nanozyme shows enzymatic activity 37.2 times than that of laccase and 13.6 times than that of a CuII 3 –MOF analogue, retains high chemical stability from pH 4 to 12, and lowers production cost by 16.2-fold relative to natural laccase. DFT calculations attribute the superior performance to the introduction of Cu+ , which yields a more favorable electronic structure, reaction energy landscape, and intermediate binding than in CuII 3 –MOF. Consequently, CuI 0.6 CuII 2.4 –MOF efficiently degrades phenolic pollutants and enables colorimetric discrimination and detection of 11 phenols as a colorimetric sensor array. It also affords sensitive detection of epinephrine and dopamine, with limits of detection of 6.5 and 10 µm , respectively. Overall, this work demonstrates a laccase-inspired route to high-activity, low-cost MOFs with strong potential for environmental remediation and biosensing. 1 Introduction Trinuclear copper complexes have garnered significant atten- tion due to their relevance in biological systems, particularly in enzymes like multicopper oxidases (MCOs), which catalyze crucial reactions such as the reduction of oxygen to water [ 1–4 ]. These enzymes, found in various organisms, are vital for the oxidative transformation of substrates and play central roles in several biochemical processes. Mimicking the structure and function of these natural systems has become a key goal in bioinorganic chemistry, offering the potential to develop effi- cient, non-precious metal catalysts for applications in biosensing,© 2026 Wiley-VCH GmbH Small Methods , 2026; 0:e02191 https://doi.org/10.1002/smtd.202502191pharmaceutical diagnostics, therapy, and ecological monitoring [ 5–10 ]. Designing trinuclear copper catalysts that mimic oxidase enzymes presents significant challenges, primarily due to the need to position three copper ions at closely spaced distances (typically less than 5 Å), allowing simultaneous interaction with O2 and the oxidizing substrate [ 11 ]. The repulsive forces between copper ions in solution complicate their close assembly during synthesis. Achieving such sub-5 Å Cu ⋅⋅⋅Cu separations is technically demanding because strong electrostatic repulsion between Cu centers often prevents their stable assembly at1 of 13 23669608, 0, D ow nloaded from https://onlinelibrary.w iley.com /doi/10.1002/sm td.202502191 by D onald T rum p - U niversity O f B ritish C olum bia , W iley O nline L ibrary on [06/01/2026]. See the T erm s and C onditions (https://onlinelibrary